Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

Cat.#:

JL53541

Regulatory Status:

RUO

Alternative:

BP8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP1; YB1; Nuclease Sensitive Element Binding Protein 1; CCAAT-binding transcription factor I subunit A; DNA-binding protein B

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.073ng/mL

Specificity:

This kit recognizes Human YBX1 in samples. No significant cross-reactivity or interference between Human YBX1 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Epigenetics

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| YBX1

Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that is encoded by the YBX1 gene.YBX1 is an RNA binding protein[6] that stabilises messenger RNAs modified with N6-methyladenosine.YBX1 is a potential drug target in cancer therapy.YB-1 helps the replication of adenovirus type 5, a commonly used vector in gene therapy.Thus, YB-1 can cause an "oncolytic" effect in YB-1 positive cancer cells treated with adenoviruses.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.01
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human YBX1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Human YBX1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	92	93
1 : 4	Range (%)	89-103	90-108
1 : 4	Average (%)	94	98